Method Training Workshops

Theoretical and hands-on experience methodological training will provide basic knowledge, background, application fields, protocols and valuable tips taught by specialists. Depending on their individual research program, MB Train fellows will select one-week training courses from the yearly program to complement their own methodological profile and to cover the entire spectrum of molecular-biochemical, biophysical and cellular biology.
At least three method training courses reflecting the interdisciplinary areas of the CRC (X-ray crystallography, protein mass spectrometry, Fluorescence imaging, Liquid and solid state NMR, Structural Bioinformatics, Single Molecule Spectroscopy and Nanoscopy, Cell biology, etc.) should be attended.

Method Training Courses SS 2018

"Production of synthetic membranes in the form of giant unilamellar vesicles (GUVs) and interaction studies of recombinant proteins with GUVs "
Sven Gould (Molekulare Evolution) / Johannes Hegemann (Funktionelle Genomforschung der Mikroorganismen)
Thorsten Eierhoff, Jan Galle , Philipp Hanisch

Requirements: No previous experience is required
Objectives:
Theoretical: first approach to the topic of synthetic membranes,
practical: learning basic techniques for the production of GUVs.
Description:
Participants will learn various techniques for making GUVs, as an example of a synthetic membrane model. Using various membrane/ lipid interacting proteins, membrane binding, dynamics, curvature, pore formation, and lipid translocation in GUVs will be studied by confocal microscopy. If possible, participants should also bring their own labeled and functional (!) Proteins (e.g., Alexa488 or fluorescein labeled). Further, it would be helpful to have information about possible lipid interaction partners (e.g., via lipid strip testing).
Dates: 3 days, 9.-11. October 2018
Registration: eierhoff@hhu.de or Jan.Galle@hhu.de
Registration deadline: 21.09.2018
Number of participants: max. 6
Location: 25.12/00.32 and 25.02/00.21

 

Special Mercartor Workshop:
" Nutrient transport regulation" & " Publishing research and research possibilities in Japan"

" Nutrient transport regulation"

- theoretical modeling
- protein translation regulation
- nutrient sensing approaches

" Publishing research and research possibilities in Japan"
- research publication, publication procedures and analysis of an example
- Japanese research infrastructure and funding

Registration via SFB 1208 office (email)

Working with eukaryotic microorganisms (Hands-on course using mainly S. cerevisiae as a model; basic methods course for low level of experience in this field) Dr. Schipper, Dr. Pohlmann, Prof. Dr. Feldbrügge Duration and dates: 3 days, 27.08., 03.09. and 10.09. Location: HHU, Institute for Microbiology Number of participants: max. 12 Registration via SFB office Registration deadline: 03.08.2018


NMR-Workshop
Dr. Philipp Neudecker
This course will give theoretical and practical insights into solution NMR spectroscopy.
No previous experience is required.
* Note that part (three days) of the course will be shared with M4414.
Therefore, the course will be given in german.
Dates: Monday 09.07.2018 - Friday 13.07.2018 , 9 a.m. - 5:30 p.m.
Registration: sfb1208(at)hhu.de
Registration deadline: 29.6.2018
Number of participants: 3
Location: FZ Jülich (Shuttle Bus)


"Gene expression, RT-qPCR"

Prof. Petra Bauer
This course addresses to persons that intend using RT-qPCR in their project.
No previous experience is required.
You will learn how to use RT-qPCR and how to analyze and present gene expression data.
* Note that this course is part of M4450.
There is the possibility to participate in the theoretical part and analyze own cDNA samples and qPCR data during or after the course on a flexible basis.
In this case it is not necessary to be present during the week the whole days.

Dates:
Monday 25.06.2018 - Friday 29.06.2018 , 9 a.m. - 5 p.m.
Registration: petra.bauer(at)hhu.de
Registration deadline: 20.6.2018
Number of participants:  4
Location: 26.13.02.11

Method Training Courses WS 2017/18

Mercartor Module - Part 2 (practical module)
"Plant Biophysics - Membrane Biophysics"
given by our Mercartor Fellow Prof. Ingo Dreyer, University of TALCA, Chile
Hands-on example for the computational simulation of transport processes; simulations using "Virtual Cell"
08.01. - 12.01.2018, 9 a.m., 26.23.00.26


Mercartor Module - Part 1

"Plant Biophysics - Membrane Biophysics"
given by our Mercartor Fellow Prof. Ingo Dreyer, University of TALCA, Chile
18.12.2017, 9 a.m., 26.23.00.26
19.12.2017, 1:00 p.m., 26.23.00.26


Content of the course in Membrane Biophysics:
· Thermodynamic bases of membrane transport processes
· Ion channels, coupled transporters, pumps
· Experimental techniques for electrophysiology
· Mathematical description of transport processes for computational modeling
· Dynamics of coupled transport processes
· Examples of successful research projects in the addressed fields

CAi course "Image analysis (Fiji / Image J) - beginner course"

Dr. Zobel, Dr. Weidtkamp-Peters, Dr. Hänsch

No previous experience with Fiji is required. You will learn how to use Fiji and how to analyze
your microscope images e. g. automatic object detection and counting as well as batch processing.
It will be possible to analyze your own images during the course.

Dates Beginner Course:
Tuesday 28.11.2017 , 9 a.m. - 5 p.m.
Thursday 30.11.2017, 9 a.m. - 5 p.m.
Registration: SFB office (email)
Registration deadline: 23.11.2017
Number of participants: max. 30
Location: t.b.a.


CAi course "Image analysis (Fiji / Image J) - advanced course"
Dr. Zobel, Dr. Weidtkamp-Peters, Dr. Hänsch

The second course is aimed at advanced users.
This course will deal with advanced plugins and macro programming.
It will be possible to analyze your own images / write your own macros during the course.

Dates Advanced course:
Monday 04.12.2017, 9 a.m. - 5 p.m.
Tuesday 05.12.2017, 9 a.m. - 5 p.m.
Registration: SFB office (email)
Registration deadline: 23.11.2017
Number of participants: max. 30
Location: t.b.a.


Mass spectrometry "Data Analysis"
Prof. Kai Stühler / Dr. Daniel Waldera-Lupa
Duration and dates: 2 days; 26.-27.09.2017, 10:00-17:00
Location: HHU, 25.41.00.61
Numer of participants: max. 18
Registration: Daniel Waldera-Lupa

X-ray course
(shifted from May to October/November 2017)
Prof. Groth / Prof. Schmitt
Duration and dates: t.b.a.
Location: HHU, Institute of Biochemistry and Institute of Biochemical Plant Physiology
Number of participants: min. 8, max. 15
Registration: SFB office (email)
Registration deadline: t.b.a.

Method Training Courses SS 2017


Advanced Imaging Methods – Superresolution
(Prerequisite: participation in "„Basic Confocal Imaging“)
Dr. Weidtkamp-Peters, Dr. Hänsch, Dr. Zobel
Duration and dates: 3 days, 04.-06.04.2017
Location: HHU, CAi
Number of participants: max. 6


Lecture Module
, overview and discussion of different subjects
2 days, 9 a.m. - 3 / 5 p.m.
Monday, May, 29th
9-11 a.m.  Georg Groth- "Protein purification"
13-15 a.m. Lutz Schmitt - "Non-natural amino acids"
Tursday, May, 30th
9 -11 a.m. Kerstin Schipper / Michael Feldbrügge - "Virtual cloning using CloneManager"
13 - 15 a.m. Andreas Weber  - "Metabolomics/Mass Spectrometry of Small Molecules"
15 - 17 a.m. Karin Meierhoff - "Mutations in plants"

Mercartor Module
Crash Kurs "Plant Biophysics - Membrane Biophysics"
given by our Mercartor Fellow Prof. Ingo Dreyer, University of TALCA, Chile
28.07.2017, 9 a.m., 26.23.00.26
31.07.2017, 1:30 p.m., 26.23.00.26


Working with eukaryotic microorganisms
(Hands-on course using mainly S. cerevisiae as a model; basic methods course for low level of experience in this field)
Dr. Schipper, Dr. Pohlmann, Prof. Dr. Feldbrügge
Duration and dates: 3 days, 04.09., 11.09. and 18.09.2017
Location: HHU, Institute for Microbiology
Number of participants: max. 12
Registration via SFB office
Registration deadline: 04.08.2017

Method Training Courses WS 16/17

 

Bioinformatics and Biomolecular Modelling
Prof. Birgit Strodel / Dr. Bogdan Barz
Duration and dates: 3 days; 28.11.-01.12.2016, 09:00-17:00
Location: HHU, 26.42.03.39
Number of participants: min. 3, max. 8
Registration deadline: 02.11.2016

Mass spectrometry and the pitfalls in sample preparation
Prof. Kai Stühler / Dr. Daniel Waldera-Lupa
Duration and dates: 1 day; 14.12.2016, 10:00-16:00
Location: HHU
Registration deadline: 18.11.2016

Basic Confocal Imaging
Dr. Weidtkamp-Peters, Dr. Hänsch, Dr. Zobel
Duration and dates: 2 days, 14.-15.2.2017
Location: HHU, CAi
Number of participants: max. 6
(You can work on your own samples.)

Basic Confocal Imaging
Dr. Weidtkamp-Peters, Dr. Hänsch, Dr. Zobel
Duration and dates: 2 days, 21.-22.2.2017
Location: HHU, CAi
Number of participants: max. 6
(You can work on your own samples.)

Advanced Imaging Methods – Superresolution
(Prerequisite: participation in "„Basic Confocal Imaging“)
Dr. Weidtkamp-Peters, Dr. Hänsch, Dr. Zobel
Duration and dates: 3 days, 28.-30.03.2017
Location: HHU, CAi
Number of participants: max. 6

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