Francesca Bottanelli, Freie Universität Berlin, Germany, will give a talk about "Investigating cellular logistics with live-cell STED super-resolution microscopy"

Abstract
In the lab we use gene editing and live-cell super-resolution microscopy to study the mechanisms of membrane homeostasis in health and disease. To be able to image physiological molecular processes in the crowded cellular cytoplasm in living cells, we developed labelling methods for stimulated emission depletion (STED) super-resolution imaging in living cells and a pipeline for the rapid generation of CRISPR-Cas9 knock ins. These methods unlocked the possibility to image dynamics at sub-50 nm spatial resolution and under near-native cellular conditions. Dynamic nanoscale microscopy of endogenously tagged machinery is revealing novel cellular roles for ARF GTPases, one of the major family of regulators of cellular membrane homeostasis. Defining the role of ARFs led to the discovery of unexplored sorting mechanisms between the endoplasmic reticulum and the Golgi apparatus. We show that bi-directional transport between the ER and the Golgi apparatus occur via a dynamic and quickly remodelling network of connecting nano-tunnels. In a similar way, Golgi-derived sorting tubules act as a sorting station between Golgi and endo-lysosomal organelles to coordinate secretory and lysosomal trafficking.

Francesca Bottanelli is assistant professor at the Institute of Chemistry and Biochemistry at Freie Universität Berlin since 2018. Her research focuses on cell biology and super-resolution imaging.
She completed her Bachelor's and Master's degrees at the University of Milan (IT), while her Master's thesis, as well as later her PhD, was done at the University of Leeds (UK). From 2011-2018, she was a post-doctoral researcher at Yale University School of Medicine. Department of Cell Biology (USA). She has received international grants for both her PhD and postdoctoral research.
In May 2021, Francesca Bottanelli was awarded a prestigious Early Career Grant under the Human Frontier Science Program (HFSP).

Host: Petra Bauer

Guests are welcome!

Thursday, June 2nd, 2022
4:30 p.m..
HS 6K

Meeting-Link: hhu.webex.com/hhu/j.php

Meeting-Kennnummer: 2732 271 3225
Passwort: FwZC89P5JW2