2020

Sanwald JL, Poschmann G, Stühler K, Behrends C, Hoffmann S, Willbold D
The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.
Cells (2020) 9, 1468

Schepers B and Gohlke H
AMBER-DYES in AMBER: Implementation of fluorophore and linker parameters into AmberTools.
J Chem Phys (2020) 152, 221103

Reindl M, Stock J, Hussnaetter KP, Genc A, Brachmann A, Schipper K
A novel factor essential for unconventional secretion of chitinase Cts1.
Frontiers in Microbiology-Fungi and Their Interactions (2020), accepted

Löwe M, Kalacheva M, Boersma AJ, Kedrov A
The more the merrier: Effects of macromolecular crowding on the structure and dynamics of biological membranes.
FEBS J (2020), doi.org/10.1111/febs.15429

Dobner J, Simons IM, Rufinatscha K, Hänsch S, Schwarten M, Weiergräber OH, Abdollahzadeh I, Gensch T, Bode JG, Hoffmann S, Willbold D
Deficiency of GABARAP but not its Paralogs Causes Enhanced EGF-induced EGFR Degradation.
Cells (2020) 9, 1296

Hoppen C and Groth G
Novel insights into the transfer routes of the essential copper cofactor to the ethylene plant hormone receptor family.
Plant Signal Behav (2020) 15(2):1716512. doi: 10.1080/15592324.2020.1716512

Hofmann A, Müller S, Drechsler T, Berleth M, Caesar K, Rohr L, Harter K, Groth G
High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1
Plants (2020) 9, 304. doi:10.3390/plants9030304

Hänsch S, Spona D, Murra G, Köhrer K, Subtil A, Furtado AR, Lichtenthaler SF, Dislich B, Mölleken K, Hegemann JH
Chlamydia-induced curvature of the host-cell plasma membrane is required for infection
Proc Natl Acad Sci U S A (2020)  pii: 201911528. doi: 10.1073/pnas.1911528117

Knopp MR, Garg SG, Handrich M, Gould SB
Major changes in plastid protein import and the origin of the Chloroplastida.
iScience (2020) 23(3):100896. doi: 10.1016/j.isci.2020.100896. [Epub ahead of print]

Steffens N, Beuter-Gunia C, Kravets E, Reich A, Legewie L, Pfeffer K, Degrandi D
Essential Role of mGBP7 for Survival of Toxoplasma gondii Infection.
mBio. (2020) 11(1), pii: e02993-19. doi: 10.1128/mBio.02993-19.

Verma N, Dollinger P, Kovacic F, Jaeger K-E, Gohlke H
The membrane-integrated steric chaperone Lif facilitates active site opening of Pseudomonas aeruginosa lipase A.
J. Comp. Chem. (2020) 41: 500-512

Mulnaes D, Porta N, Clemens R, Apanasenko I, Reiners J, Gremer
L, Neudecker P, Smits SHJ, Gohlke H
TopModel: Template-based protein structure prediction at low sequence identity using top-down consensus and deep neural networks.
J. Chem. Theory Comput. (2020). doi: 10.1021/acs.jctc.9b00825. [Epub ahead of print]

Gratz R, Brumbarova T, Ivanov R, Trofimov K, Tünnermann L, Ochoa-Fernandez R, Blomeier T, Meiser J, Weidtkamp-Peters S, Zurbriggen M, Bauer P
Phospho-mutant activity assays provide evidence 1 for alternative phospho-regulation pathways of the transcription factor FIT
New Phytol. (2020) 225(1): 250-267. doi: 10.1111/nph.16168. [Epub 2019 Oct 13]

2019

Trofimov K, Ivanov R, Eutebach M, Acaroglu B, Mohr I, Bauer P, Brumbarova T
Mobility and localization of the iron deficiency-induced transcription factor bHLH039 change in the presence of FIT.
Plant Direct. (2019) 3(12):e00190. doi:10.1002/pld3.190

Legewie L, Loschwitz J, Prescher M, Steffens N, Wang X, Smits SHJ, Schmitt L, Strodel B, Degrandi D, Pfeffer K
Biochemical and structural characterization of murine GBP7, a guanylate biniding protein with an unconventional C-terminal tail.
Biochem. J. (2019) 476(21): 3161-3182

Galle JN, Fechtner T, Eierhoff T, Römer W, Hegemann JH
A Chlamydia pneumoniae adhesin induces phosphatidylserine exposure on host cells.
Nat Commun (2019) 10(1):4644. doi: 10.1038/s41467-019-12419-8

Denay G, Schultz P, Haensch S, Weidtkamp-Peters S, Simon R
Over the rainbow: a practical guide for fluorescent protein selection in plant FRET experiments
Plant Direct. (2019) 3(12):e00189. doi: 10.1002/pld3.189

Barz B, Loschwitz J, Strodel B
Large-scale, dynamin-like motions of the human guanylate binding protein 1 revealed by multi-resolution simulations
PLoS Comput Biol. (2019) 7;15(10):e1007193. doi: 10.1371/journal.pcbi.1007193

Galle JN, Hegemann JH
Exofacial phospholipids at the plasma membrane: ill-defined targets for early infection processes.
Biol. Chem. (2019) 400(10):1323-1334. doi: 10.1515/hsz-2019-0187

Breda AS, Hazak O, Schultz P, Anne P, Graeff M, Simon R, Hardtke CS
A Cellular Insulator against CLE45 Peptide Signaling
Curr. Biol. (2019) 29, 1-8. doi.org/10.1016/j.cub.2019.06.037

Röder C, Vettore N, Mangels LN, Gremer L, Ravelli RBG, Willbold D, Hoyer W, Buell AK, Schröder GF
Atomic structure of PI3-kinase SH3 amyloid fibrils by cryo-electron microscopy.
Nat Commun. (2019) 10(1):3754. doi: 10.1038/s41467-019-11320-8

Kanonenberg K, Royes J, Kedrov A, Poschmann G, Angius F, Solgadi A, Spitz O, Kleinschrodt D, Stühler K, Miroux B, Schmitt L
Shaping the lipid composition of bacterial membranes for membrane protein production.
Microbial Cell Factories (2019), 18:131 doi 10.1186/s12934-019-1182-1

Krichel C, Möckel C, Schillinger O, Huesgen PF, Sticht H, Strodel B, Weiergräber OH, Willbold D, Neudecker P
Solution structure of the autophagy-related protein LC3C reveals a polyproline II motif on a mobile tether with phosphorylation site.
Sci Rep. (2019) 9(1):14167. doi: 10.1038/s41598-019-48155-8

Handrich MR, Garg SG, Sommerville EW, Hirt RP, Gould S
Characterization of the BspA and Pmp protein family of trichomonads
Parasit Vectors (2019) 12(1):406. doi: 10.1186/s13071-019-3660-z.

Berleth M, Berleth N, Minges A, Hänsch S, Burkart RC, Stork B, Stahl Y, Weidtkamp-Peters S, Simon R, Groth G
Molecular Analysis of Protein-Protein Interactions in the Ethylene Pathway in the Different Ethylene Receptor Subfamilies.
Front. Plant Sci. (2019) 10:726. doi: 10.3389/fpls.2019.00726

Bauemers M, Klose S, Brueser C, Haag C, Haensch S, Pannen H, Weidtkamp-Peters S, Feldbrügge M, Klein T
The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms.
Biol. Open (2019) 8, bio043422. doi:10.1242/bio.043422

Shanmugarajah K, Linka N, Gräfe K, Smits SHJ, Weber APM, Zeier J, Schmitt L
ABCG1 contributes to suberin formation in Arabidopsis thaliana roots.
Sci. Rep. (2019) 9(1):11381. doi: 10.1038/s41598-019-47916-9

Kater L, Frieg B, Berninghausen O, Gohlke H, Beckmann R, Kedrov A
Partially inserted nascent chain unzips the lateral gate of the Sec translocon.
EMBO Rep. (2019) 20(10):e48191. doi: 10.15252/embr.201948191

Hoppen C, Müller L, Hänsch S, Uzun B, Milić D, Meyer AJ, Weidtkamp-Peters S, Groth G
Soluble and membrane-bound protein carrier mediate direct copper transport to the ethylene receptor family
Sci. Rep. (2019) 9(1): 10715. doi: 10.1038/s41598-019-47185-6

Schott-Verdugo S, Müller L, Classen E, Gohlke H, Groth G
Structural model of the ETR1 ethylene receptor transmembrane sensor domain.
Sci. Rep. (2019)  9(1):8869. doi: 10.1038/s41598-019-45189-w

Jankowski S, Pohlmann T, Baumann S, Müntjes K, Devan SK, Zander S, Feldbrügge M
The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.
EMBO Rep. (2019) 20(9):e47381. doi: 10.15252/embr.201847381

Müller J, Pohlmann T, Feldbrügge M
Core components of endosomal mRNA transport are evolutionarily conserved in fungi.
Fungal Genet. Biol. (2019) 126: 12-16 

Klinker S, Stindt S, Gremer L, Bode JG, Gertzen CGW, Gohlke H, Weiergräber OH, Hoffmann S, Willbold D
Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.
J Biol Chem. (2019) 294(18):7388-7402

Schott-Verdugo S, Gohlke H
PACKMOL-Memgen: A simple-to-use generalized workflow formembrane-protein/lipid-bilayer system building.        
J. Chem. Inf. Model. (2019) 59(6):2522-2528. doi: 10.1021/acs.jcim.9b00269

Kanonenberg K, Smits S, Schmitt, L
Functional Reconstitution of HlyB, a Type I Secretion ABC Transporter, in Saposin-A Nanoparticles
Sci. Rep. (2019) 9(1):8436. doi: 10.1038/s41598-019-44812-0

Béthune J, Jansen R-P, Feldbrügge M, Zarnack K
Membrane-associated RNA-binding proteins orchestrate organelle-coupled translation.
Trends Cell Biol. (2019) 29: 178-188

Abdollahzadeh I, Hendriks J, Sanwald JL, Simons IM, Hoffmann S, Weiergräber OH, Willbold D, Gensch T
Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging.
Molecules (2019) 24(9). pii: E1833. doi: 10.3390/molecules24091833.

Khan I, Gratz R, Denezhkin P, Schott-Verdugo S, Angrand K, Genders L, Basgaran R, Fink-Straube C, Brumbarova T, Gohlke H, Bauer P, Ivanov R
Calcium-promoted interaction between the C2-domain protein EHB1 and metal transporter IRT1 inhibits Arabidopsis iron acquisition
Plant Physiol. (2019) 180(3):1564-1581. doi: 10.1104/pp.19.00163.

Schwarten M, Weiergräber OH, Petrović D, Strodel B, Willbold D
Structural Studies of Autophagy-Related Proteins.
Methods Mol Biol (2019) 1880: 17-56. doi: 10.1007/978-1-4939-8873-0_2

Spitz O, Erenburg IN, Beer T, Kanonenberg K, Holland IB, Schmitt L
Type I secretion systems - One mechanism for all?
Microbiol Spectr. (2019) 7(2). doi: 10.1128/microbiolspec.PSIB-0003-2018.

Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger K-E, Kovacic F
Expression, purification and crystallization of Pseudomonas aeruginosa
esterase PA2949, a bacterial homologue of human membrane esterase ABHD6.
Acta Cryst. F (2019) 75, 270-277

Werthebach M, Stewart FA, Gahlen A, Mettler-Altmann T, Akhtar I, Maas-Enriquez K, Droste A, Eichmann TO, Poschmann G, Stühler K, Beller M
Control of Drosophila Growth and Survival by the Lipid Droplet-Associated Protein CG9186/Sturkopf.
Cell Rep (2019) 26(13):3726-3740.e7. doi: 10.1016/j.celrep.2019.02.110.

Hoppen C, Müller L, Albrecht AC, Groth G
The NOP-1 peptide derived from the central regulator of ethylene signaling EIN2 delays floral senescence in cut flowers.
Sci Rep (2019) 9(1):1287. doi: 10.1038/s41598-018-37571-x.

Zavřel T, Faizi M, Loureiro C, Poschmann G, Stühler K, Sinetova M, Zorina A, Steuer R, Červený J
Quantitative insights into the cyanobacterial cell economy.
Elife (2019) 8;pii: e42508. doi: 10.7554/eLife.42508.

Reindl M, Hänsch S, Weidtkamp-Peters S, Schipper K
A Potential Lock-Type Mechanism for Unconventional Secretion in Fungi.
Int. J. Mol. Sci. (2019) 20(3):460. doi: 10.3390/ijms20030460

Simons IM, Mohrlüder J, Feederle R, Kremmer E, Zobel T, Dobner J, Bleffert N, Hoffmann S, Willbold D    
The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels.
Sci Rep. (2019) 9(1):526. doi: 10.1038/s41598-018-36717-1

Dreyer I, Spitz O, Kanonenberg K, Montag K, Handrich M, Ahmad S, Schott-Verdugo S, Navarro-Retamal C, Rubio-Meléndez M, Gomez-Porras JL, Riedelsberger J, Molina-Montenegro M, Succurro A, Zuccaro A, Gould S, Bauer P, Schmitt L, Gohlke H
Nutrient exchange in arbuscular mycorrhizal symbiosis from a thermodynamic point of view.
New Phytol. (2019) 222: 1043-1053

Haag C, Klein T, Feldbrügge M (2019)
Functional in vivo analysis of ESCRT components using model fungi.
In: Culetto E., Legouis R. (eds) The ESCRT Complexes. Methods in Molecular Biology, vol 1998, pp 251-271. Humana, New York, NY; DOI: 10.1007/978-1-4939-9492-2_19

2018

Möckel C, Kubiak J, Schillinger O, Kuehnemuth R, Della Corte D, Schröder GF, Willbold D, Strodel B, Seidel CAM, Neudecker P
Integrated NMR, Fluorescence and MD Benchmark Study of Protein Mechanics and Hydrodynamics.
J. Phys. Chem. B (2018) 123: 1453-1480

Aschenbroich J, Hussnaetter KP, Stoffels P, Langner T, Zander S, Sandrock B, Bölker M, Feldbrügge M, Schipper K
The germinal Centre kinase Don3 is crucial for unconventional secretion of chitinase Cts1 in Ustilago maydis.
Biochim. Biophys. Acta Proteins Proteom. (2018) 123: 1453-1480

Schnute B, Troost T, Klein T
Endocytic Trafficking of the Notch Receptor.
Adv Exp Med Biol. (2018) 1066:99-112, doi: 10.1007/978-3-319-89512-3_6

Mulnaes D and Gohlke H
TopScore: Using deep neural networks and large diverse data sets for accurate protein model quality assessment.
J. Chem. Theory Comput. (2018) 14: 6117-6126

Rösener NS, Gremer L, Reinartz E, König A, Brener O, Heise H, Hoyer W, Neudecker P, Willbold D
A D-enantiomeric peptide interferes with heteroassociation of amyloid-β oligomers and prion protein.
J. Biol. Chem. (2018) 293: 15748-15764

Ökmen B, Kemmerich B, Hilbig D, Wemhöner R, Aschenbroich J, Perrar A, Huesgen PF, Schipper K, Doehlemann G
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis.
New Phytol. (2018) 220: 249-261

Owen MC, Kulig W, Rog T, Vattulainen I, Strodel B
Cholesterol Protects the Oxidized Lipid Bilayer from Water Injury: An All-Atom Molecular Dynamics Study.
J. Membr. Biol. (2018) 251: 521-534

Monné M, Daddabbo L, Gagneul D, Obata T, Hielscher B, Palmieri L, Miniero DV, Fernie AR, Weber APM, Palmieri F
Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate and dicarboxylates.
J. Biol. Chem. (2018) 293: 4213-4227

Kanonenberg K, Spitz O, Erenburg I, Beer T, Schmitt L
Type I secretion system - it takes three and a substrate.
FEMS Microbiol. Lett. (2018) 365(11). doi: 10.1093/femsle/fny094

Preisner H, Habicht J, Garg SG, Gould SB
Intermediate filament protein evolution and protists.
Cytoskeleton (2018) 75: 231-243

Milić D, Dick M, Mulnaes D, Pfleger C, Kinnen A, Gohlke H, Groth G
Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1.
Sci. Rep. (2018) 8: 3890. doi: 10.1038/s41598-018-21952-3

de Vries J and Gould SB
The monoplastidic bottleneck in algae and plant evolution.
J. Cell Sci. (2018) 131: jcs203414. doi: 10.1242/jcs.203414

Zander S, Müntjes K, Feldbrügge M
RNA live imaging in the model microorganism Ustilago maydis.
Methods Mol. Biol. (2018) RNA detection, ed. I. Gaspar, Humana Press, New York, 1649: 319-335.

Nowack ECM and Weber APM
Genomics-informed insights into endosymbiotic organelle evolution in photosynthetic eukaryotes.
Annu. Rev. Plant Biol. (2018) 69: 51-84

Heucken N and Ivanov R
The retromer, sorting nexins and the plant endomembrane protein trafficking.
J. Cell Sci. (2018) 131(2). pii: jcs203695. doi: 10.1242/jcs.203695

2017

Volkov O, Kovalev K, Polovinkin V, Borshchevskiy V, Bamann C, Astashkin R, Marin E, Popov A, Balandin T, Willbold D, Büldt G, Bamberg E, Gordeliy V
Structural insights into ion conduction by channelrhodopsin 2.
Science (2017) 358(6366), eaan8862. doi: 10.1126/science.aan8862

Waldera-Lupa DM, Etemad-Parishanzadeh O, Brocksieper M, Kirchgaessler N, Seidel S, Kowalski T, Montesinos-Rongen M, Deckert M, Schlegel U, Stühler K
Proteomic changes in cerebrospinal fluid from primary central nervous system lymphoma patients are associated with protein ectodomain shedding.
Oncotarget (2017) 8(66): 110118-110132

Berndt N, Seib E, Kim S, Troost T, Lyga M, Langenbach J, Haensch S, Kalodimou K, Delidakis C, Klein T
Ubiquitylation-independent activation of Notch signalling by Delta.
Elife. (2017) 6: e27346. doi: 10.7554/eLife.27346

Kessenbrock M, Klein SM, Müller L, Hunsche M, Noga G, Groth G
Novel Protein-Protein Inhibitor Based Approach to Control Plant Ethylene Responses: Synthetic Peptides for Ripening Control.
Front. Plant Sci. (2017) 8: 1528. doi: 10.3389/fpls.2017.01528

Lindenberg V, Mölleken K, Kravets E, Stallmann S, Hegemann JH, Degrandi D, Pfeffer K
Broad recruitment of mGBP family members to Chlamydia trachomatis inclusions.
PLoS One (2017) 12(9): e0185273. doi: 10.1371/journal.pone.0185273

Abdollahzadeh I, Schwarten M, Gensch T, Willbold D, Weiergräber OH
The Atg8 Family of Proteins-Modulating Shape and Functionality of Autophagic Membranes.
Front. Genet. (2017) 8: 109. doi: 10.3389/fgene.2017.00109

Mölleken K and Hegemann JH
Acquisition of Rab11 and Rab11-Fip2 - A novel strategy for Chlamydia pneumoniae early survival.
PLoS Pathog. (2017) 13(8): e1006556. doi: 10.1371/journal.ppat.1006556

Singer A, Poschmann G, Mühlich C, Valadez-Cano C, Hänsch S, Hüren V, Rensing SA, Stühler K, Nowack ECM
Massive protein import into the early evolutionary stage photosynthetic organelle of the amoeba Paulinella chromatophora.
Curr. Biol. (2017) 27: 2763-2773

Boeske A, Schwarten M, Ma P, Tusche M, Mötter J, Möller C, Neudecker P, Hoffmann S, Willbold D
Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.
Sci. Rep. (2017) 7: 5979. doi: 10.1038/s41598-017-06319-4

Hartings S, Paradies S, Karnuth B, Eisfeld S, Mehsing J, Wolff C, Levey T, Westhoff P, Meierhoff K
The DnaJ-like zinc-finger protein HCF222 is required for thylakoid membrane biogenesis.
Plant Physiol. (2017) 174: 1807-1824

Mergaert P, Kikuchi Y, Shigenobu S, Nowack ECM
Metabolic Integration of bacterial endosymbionts through antimicrobial peptides.
Trends Microbiol. (2017) 25: 703-712

McMillan B, Tibbe C, Drabek A, Seegar T, Blacklow S, Klein T
Structural basis for regulation of ESCRT-III complexes by Lgd.
Cell Rep. (2017) 19: 1750-1757

Martin WF, Tielens AGM, Mentel M, Garg SG, Gould SB
The physiology of phagocytosis in the context of mitochondrial origin.
Microbiol. Mol. Biol. Rev. (2017) 81(3): e00008-17. doi: 10.1128/MMBR.00008-17

Haag C, Pohlmann T, Feldbrügge M
The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.
PLoS Genet. (2017) 13(4):e1006734. doi: 10.1371/journal.pgen.1006734

Terfrüchte M, Reindl M, Jankowski S, Sarkari P, Feldbrügge M, Schipper K
Applying unconventional secretion in Ustilago maydis for the export of functional nanobodies.
Int. J. Mol. Sci. (2017) 18, 937. doi: 10.3390/ijms18050937

Lundquist PK, Mantegazza O, Stefanski A, Stühler K, Weber APM
Surveying the Oligomeric State of Arabidopsis thaliana Chloroplasts.
Mol. Plant. (2017) 10: 197-211.

Ivanov R and Bauer P
Sequence and coexpression analysis of iron-regulated ZIP transporter genes reveals crossing points between iron acquisition strategies in green algae and land plants.
Plant Soil (2017) 418: 61-73 2016

2016

Reimann S, Poschmann G, Kanonenberg K, Stühler K, Smits SH, Schmitt L
Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli.
Biochem. J. (2016) 473: 2471-2483

Fechtner T, Galle JN, Hegemann JH
The novel chlamydial adhesin CPn0473 mediates the lipid raft-dependent uptake of Chlamydia pneumoniae.
Cell Microbiol. (2016) 18(8): 1094-1105

Ball SG, Bhattacharya D, Weber APM
Pathogen to powerhouse. How did the precursors to the mitochondrion and the plastid evade host defense?
Science (2016) 351(6274): 659-660

Gould SB
Infection and the first eukaryotes.
Science (2016) 352(6289): 1065

Garg S and Gould SB
The role of charge in protein targeting evolution.
Trends Cell Biol. (2016) 26(12): 894-905

Lenders MHH, Beer T, Smits SHJ, Schmitt L
In vivo quantification of the secretion rates of the hemolysin A Type I secretion system.
Sci. Rep. (2016) 6: 33275. doi: 10.1038/srep33275

Preisner H, Karin EL, Poschmann G, Stühler K, Pupko T, Gould SB
The cytoskeleton of parabasalian parasites comprises proteins that share properties common to intermediate filament proteins.
Protist (2016) 167: 526-543

McMillan BJ, Tibbe C, Jeon H, Drabek AA, Klein T, Blacklow SC
Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein.
Cell Rep. (2016) 16(5): 1211-1217

Brumbarova T and Ivanov R
Differential Gene Expression and Protein Phosphorylation as Factors Regulating the State of the Arabidopsis SNX1 Protein Complexes in Response to Environmental Stimuli.
Front. Plant Sci. (2016) 7: 1456

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