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Z02: In vivo fluorescence imaging - Analysis of membrane protein complexes in space and time

The Center for Advanced Imaging (CAi) at Heinrich Heine University provides state-of-the-art microscopy equipment for the in vivo analysis of membrane protein complexes in space and time. This includes systems for super-resolution fluorescence microscopy techniques like SIM (structured illumination microscopy), PALM and STORM (photoactivated localization microscopy, stochastic optical reconstruction microscopy) (Zeiss ELYRA PS), and STED microscopy (stimulated emission depletion) (Leica SP8 gSTED 3X), as well as confocal systems (Olympus FV1000, Zeiss LSM 780) with extensions for time correlated single photon counting (TCSPC) (Picoquant) to detect simultaneously multiple fluorescence parameters with high sensitivity like in FLIM (fluorescence lifetime imaging microscopy) measurements. These fluorescence microscopic approaches are complemented by transmission and scanning electron microscopy (Zeiss TEM 902, Zeiss REM Leo 1430 VP) to gain ultimate resolution. This will allow us to investigate quantitatively the signaling reactions across membranes and to analyze receptor complex compositions of different organisms under physiological conditions and at endogenous expression levels in cells and tissues. The final goal of this central project is to help to unravel general aspects of the functionality of membrane systems.

Project leader: Dr. Stefanie Weidtkamp-Peters,

Researcher: Dr. Miriam Bäumers,