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Publications

2022

Philipp M, Hussnaetter KP, Reindl M, Müntjes K, Feldbrügge M, Schipper K
A Novel Potent Carrier for Unconventional Protein Export in Ustilago maydis
Front Cell Dev Biol (2022) 9:816335; doi: 10.3389/fcell.2021.816335

Pourhassan Z, Hachani E, Spitz O, Smits S H J & Schmitt L (2022): Investigations on the substrate binding sites of hemolysin B, an ABC transporter, of a type 1 secretion system Front. Microbiol. (2022) in press

Erenburg IN, Hänsch S, Chacko FM, Hamacher A, Wintgens S, StuhldreierF, Poschmann G, Spitz O, Stühler K, Wesselborg S, Hegemann J, Smits SHJ, Weidtkamp-Peter S, Schmitt L
Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the human host cell
Sci Rep (2022) in press

Papadopoulos A, Busch M, Reiners J, Hachani E, Bäumers M, Berger J, Schmitt L, Jaeger K-E, Kovacic F, Smits SHJ, Kedrov A
The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa
Front Mol Biosci (2022); doi:10.3389/fmolb.2022.1026724

Cutsail G, Schott-Verdugo S, Müller L, DeBeer S, Groth G, Gohlke H
Spectroscopic and QM/MM studies of the Cu(I) binding site of the plant ethylene receptor ETR1
Biophys J (2022); doi:10.1016/j.bpj.2022.09.007

Lu J, Wen C-K, Groth G
New Insights into Phase Separation Processes and Membraneless Condensates of EIN2
Plants (2022) 11(16):2149; doi:10.3390/plants11162149

Narasimhan M and Rüdiger Simon R
Spatial range, temporal span, and promiscuity of CLE-RLK signaling
Front Plant Sci (2022); doi:10.3389/fpls.2022.906087

Brown H, Chung M, Üffing A, Batistatou N, Tsang T, Doskocil S, Mao W, Willbold D, Bast Jr RC, Lu Z, Weiergräber OH, Kritzer JA
Structure-Based Design of Stapled Peptides That Bind GABARAP and Inhibit Autophagy
J Am Chem Soc (2022) 144(32), 14687-14697; doi:10.1021/jacs.2c04699

Baeumers M, Schulz K, Klein T
Using Drosophila melanogaster to analyse the human paralogs of the ESCRT-III core component Shrub/CHMP4/Snf7 and its interactions with members of the LGD/CC2D1 family
Int J Mol Sci (2022) 23(14):7507; doi:10.3390/ijms23147507

Devan SK, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits SHJ, Gohlke H, Feldbrügge M
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes
PLoS Genet (2022), 18(6):e1010269; doi:10.1371/journal.pgen.1010269

Kleist TJ, Lin IW, Xu S, Maksaev G, Sadoine M, Haswell ES, Frommer WB, Wudick MM
OzTracs: optical osmolality reporters engineered from mechanosensitive ion channels
Biomolecules (2022) 12(6):787; doi:10.3390/biom12060787

Kuhnert F and Weber APM
In Vivo Epitope Tagging of Plant Mitochondria
Methods Mol Biol (2022) 2379:253-264; doi:10.1007/978-1-0716-1791-5_14

Kuhnert F and Weber APM
Isolation of Plant Mitochondria Using Affinity Purification
Methods Mol Biol (2022) 2363:25-37; doi:10.1007/978-1-0716-1653-6_3

Oberleitner L,  Perrar A, Macorano L, Huesgen PF, Nowack ECM
A bipartite chromatophore transit peptide and N-terminal protein processing in the Paulinella chromatophore
Plant Phys (2022) 189(1), 152-164; doi:10.1093/plphys/kiac012

Bleffert F, Granzin J, Caliskan M, Schott-Verdugo SN, Siebers M, Thiele B, Rahme LG, Felgner S, Dörmann P, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F
Structural, mechanistic and physiological insights into phospholipase A-mediated membrane phospholipid degradation in Pseudomonas aeruginosa
eLife (2022) 11:e72824; doi:10.7554/eLife.72824

Schreiber M, Rensing SA, Gould SB
The greening ashore
Trends Plant Sci (2022) 27(9), 847-857; doi:10.1016/j.tplants.2022.05.005. Epub 2022 Jun 20

Kugele A, Uzun B, Müller L, Schott-Verdugo S, Gohlke H, Groth G, Drescher M
Mapping the helix arrangement of the reconstituted ETR1 ethylene receptor transmembrane domain by EPR spectroscopy
RSC Adv (2022) 12(12), 7352-7356; doi:10.1039/d2ra00604a

Soldierer M, Bister A, Haist C, Thivakaran A, Cengiz SC, Sendker S, Bartels N, Thomitzek A, Smorra D, Hejazi M, Uhrberg M, Scheckenbach K, Monzel C, Wiek C, Reinhardt D, Niktoreh N, Hanenberg H
Genetic Engineering and Enrichment of Human NK Cells for CAR-Enhanced Immunotherapy of Hematological Malignancies
Front Immunol (2022) 13:847008; doi:10.3389/fimmu.2022.847008. eCollection 2022

MacLeod AI, Raval PK, Stockhorst S, Knopp MR, Frangedakis E, Gould SB
Loss of plastid developmental genes coincides with a reversion to monoplastidy in hornworts
Front Plant Sci (2022) 13:863076; doi:10.3389/fpls.2022.863076

Weiler AJ, Spitz O, Gudzuhn M, Schott-Verdugo SN, Kamel M, Thiele B, Streit WR, Kedrov A, Schmitt L, Gohlke H, Kovacic F
A phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly
Biochim Biophys Acta - Mol Cell Biol Lipids (2022) 1867(4):159101; doi:10.1016/j.bbalip.2021.159101

Pourhassan NZ, Cui H, Khosa S, Davari MD, Jaeger K-E, Smits SHJ, Schwaneberg U, Schmitt L
Optimized Hemolysin type 1 secretion system in Escherichia coli by directed evolution of the Hly enhancer fragment and including a terminator region
ChemBioChem (2022) 23:e202100702; doi:10.1002/cbic.202100702

Fatafta H, Kav B, Bundschuh BF, Loschwitz J, Strodel B
Disorder-to-order transition of the amyloid-β peptide upon lipid binding
Biophys Chem (2022) 280:106700; doi:10.1016/j.bpc.2021.106700

Castro-Rodríguez V, Kleist TJ, Gappel NM, Atanjaoui F, Okumoto S, Machado M, Denyer T, Timmermans MCP, Frommer WB, Wudick MM
Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development
Plant J (2022) 109(3), 664-674; doi:10.1111/tpj.15585.  Epub 2021 Dec 2

Beer T, Hänsch S, Pfeffer K, Smits SHJ, Weidtkamp-Peters S, Schmitt L
Quantification and surface localization of the hemolysin A type 1 secretion system at the endogenous level and under conditions of overexpression
Appl Environm Microbiol (2022) 88(3):e0189621; doi:10.1128/AEM.01896-21. Epub 2021 Dec 1

Dao O, Kuhnert F, Weber APM, Peltier G, Li-Beisson Y
Physiological functions of malate shuttles in plants and algae
Trends Plant Sci (2022) 27:488-501; doi: 10.1016/j.tplants.2021.11.007. Epub 2021 Nov 27

Kamel M, Löwe M, Schott-Verdugo S, Gohlke H, Kedrov A
Unsaturated fatty acids augment protein transport via the SecA:SecYEG translocon
FEBS Journal (2022) 289(1), 140-162; doi:10.1111/febs.16140. Epub 2021 Aug 6

2021

Tang K, Beyer HM, Zurbriggen MD, Gartner W
The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters
Chem Rev (2021) 121(24), 14906-14956; doi:10.1021/acs.chemrev.1c00194

Spitz O, Erenburg IN, Kannonenberg K, Peherstorfer S, Lenders MHH, Reiners J, Ma M, Luisi BF, Smits SHJ, Schmitt L
Identity determinants of the translocation signal for a Type 1 secretion system
Front Physiol (2021) 12:804646; doi:10.3389/fphys.2021.804646

Anand R, Reichert AS, Kondadi AK
Emerging Roles of the MICOS Complex in Cristae Dynamics and Biogenesis
Biology (Basel) (2021) 10(7):600. doi: 10.3390/biology10070600

Rigano A, Ehmsen S, Öztürk SU, Ryan J, Balashov A, Hammer M, Kirli K, Boehm U, Brown CM, Bellve K, Chambers JJ, Cosolo A, Coleman RA, Faklaris O, Fogarty KE, Guilbert T, Hamacher AB, Itano MS, Keeley DP, Kunis S, Lacoste J, Laude A, Ma WY, Marcello M, Montero-Llopis P, Nelson G, Nitschke R, Pimentel JA, Weidtkamp-Peters S, Park PJ, Alver BH, Grunwald D, Strambio-De-Castillia C
Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications
Nat Methods (2021) 18(12), 1489-1495; doi:10.1038/s41592-021-01315-z

Ahmad S, Strunk CH, Schott-Verdugo SN, Jaeger K-E, Kovacic F, Gohlke H
Substrate access mechanism in a novel membrane-bound phospholipase A of Pseudomonas aeruginosa concordant with specificity and regioselectivity
J Chem Inf Model (2021) 61(11), 5626-5643; doi:10.1021/acs.jcim.1c00973

Mulnaes, D, Schott-Verdugo S, Koenig F, Gohlke H
TopProperty: Robust meta-prediction of transmembrane and globular protein features using deep neural networks
J Chem Theory Comput (2021) 17(11), 7281-7289; doi:10.1021/acs.jctc.1c00685

Schlegel J, Denay G, Wink R, Pinto KG, Stahl Y, Schmid J, Blumke P, Simon RG
Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
Elife (2021)
10:e70934; doi: 10.7554/eLife.70934

Kunis S, Hänsch S, Schmidt C, Wong F, Strambio-De-Castillia C, Weidtkamp-Peters S
MDEmic: a metadata annotation tool to facilitate management of FAIR image data in the bioimaging community
Nat Methods (2021) 18(12), 1416-1417; doi:10.1038/s41592-021-01288-z

Fatafta H,  Khaled M, Owen MC, Sayyed-Ahmad A, Strodel B
Amyloid-β peptide dimers undergo a random coil to β-sheet transition in the aqueous phase but not at the neuronal membrane
Proc Natl Acad Sci U S A (2021) 118(39):e2106210118; doi:10.1073/pnas.2106210118

Viana MP, Levytskyy RM, Anand R, Reichert AS, Khalimonchuk O
Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex
iScience (2021) 24:102119; doi: 10.1016/j.isci.2021.102119

Novoselova IP, Neusch A, Brand J-S, Otten M, Safari MR, Bartels N, Karg M, Farle M, Wiedwald U, Monzel C
Magnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells
Nanomaterials (2021) 11(9):2267; doi:10.3390/nano11092267

Navarro-Retamal C, Schott-Verdugo S, Gohlke H, Dreyer I
Computational analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) permeation pathway
Int J Mol Sci (2021) 22(19):10345; doi:10.3390/ijms221910345

Green MN, Gangwar SP, Michard E, Simon AA, Portes MT, Barbosa-Caro J, Wudick MM, Lizzio MA, Klykov O, Yelshanskaya MV, Feijó JA, Sobolevsky AI
Structure of the Arabidopsis thaliana glutamate receptor-like channel GLR3.4
Molecular Cell (2021) 81(15), 3216–3226 e8; doi:10.1016/j.molcel.2021.05.025

Urbach J, Kondadi AK, David C, Naha R, Deinert K, Reichert AS, Anand R
Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes
Biochim Biophys Acta Biomembr (2021) 1863(12):183683. doi: 10.1016/j.bbamem.2021.183683

Wierckx N, Miebach K, Ihling N, Hussnaetter KP, Büchs J, Schipper K
Perspectives for the application of Ustilaginaceae as biotech cell factories
Essays Biochem (2021) 65(2), 365-379; doi:10.1042/EBC20200141

Moe-Lange J, Gappel NM, Machado M, Wudick MM, Sies CSA, Schott-Verdugo SN, Bonus M, Mishra S, Hartwig T, Bezrutczyk M, Basu D, Farmer EE, Gohlke H, Malkovskiy A, Haswell ES, Lercher MJ, Ehrhardt DW, Frommer WB, Kleist TJ
Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling
Sci Adv (2021) 7(37):eabg4298; doi:10.1126/sciadv.abg4298

Müntjes K, Devan SK, Reichert AS, Feldbrügge M
Linking transport and translation of mRNAs with endosomes and mitochondria
EMBO Rep (2021) 22(10):e52445; doi:10.15252/embr.202152445

Sadoine M, Ishikawa Y, Kleist TJ, Wudick MM, Nakamura M, Grossmann G, Frommer WB, Ho C-H
Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology
Plant Physiol (2021) 187(2), 485-503; doi:10.1093/plphys/kiab353

Schmitz K, Cox J, Esser LM, Voss M, Sander K, Löffler A, Hillebrand F, Erkelenz S, Schaal H, Kähne T, Klinker S, Zhang T, Nagel-Steger L, Willbold D, Seggewiß S, Schlütermann D, Stork B, Grimmler M, Wesselborg S, Peter C
An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis
Nucleic Acids Res (2021) 49(11), 6437-6455; doi:10.1093/nar/gkab452

Blümke P, Schlegel J, Gonzalez-Ferrer C, Becher S, Gustavo Pinto K, Monaghan J, Simon R
Receptor-like cytoplasmic kinase MAZZA mediates developmental processes with CLAVATA1 family receptors in Arabidopsis
J Exp Bot (2021) 72(13, :4853-4870; doi: 10.1093/jxb/erab183

Seib E and Klein T
The role of ligand endocytosis in notch signalling
Biol Cell (2021) 113(10), 401–418; doi:10.1111/boc.202100009

Kim J-Y, Loo EP-I, Pang TY, Lercher M, Frommer WB, Wudick MM
Cellular export of sugars and amino acids: role in feeding other cells and organisms
Plant Physiol (2021)  187(4), 1893-1914; doi:10.1093/plphys/kiab228

Deitersen J, Berning L, Stuhldreier F, Ceccacci S, Schlütermann D, Friedrich A, Wu W, Sun Y, Böhler P, Berleth N, Mendiburo MJ, Seggewiß S, Anand R, Reichert AS, Monti MC, Proksch P, Stork B
High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol
Cell Death Dis (2021) 12(6):560; doi:10.1038/s41419-021-03830-5

Mulnaes D, Golchin P, Koenig F, Gohlke H
TopDomain: Exhaustive protein domain boundary meta-prediction combining multi-source information and deep learning
J Chem Theory Comput (2021) 17(7), 4599-4613; doi:10.1021/acs.jctc.1c00129

Ludwig N, Reissmann S, Schipper K, Gonzalez C, Assmann D, Glatter T, Moretti M, Ma L-S, Rexer K-H, Snetselaar K, Kahmann R
A cell surface-exposed protein complex with an essential virulence function in Ustilago maydis
Nat Microbiol (2021) 6(6), 722-730; doi:10.1038/s41564-021-00896-x

Haist C, Schulte E, Bartels N, Bister A, Poschinski Z, Ibach TZ, Geipe K, Wiek C, Wagenmann M, Monzel C, Scheckenbach K, Hanenberg H
CD44v6-targeted CAR T-cells specifically eliminate CD44 isoform 6 expressing head/neck squamous cell carcinoma cells
Oral Oncology (2021) 116:105259; doi:10.1016/j.oraloncology.2021.105259

Zöllner NR, Bezrutczyk M, Laureyns R, Nelissen H, Simon R, Frommer WB
An RNA in situ hybridization protocol optimized for monocot tissue
STAR Protocols (2021) 2(2):100398; doi:10.1016/j.xpro.2021.100398

Prescher N, Hänsch S, Knobbe-Thomsen CB, Stühler K, Poschmann G
The migration behavior of human glioblastoma cells is influenced by the redox-sensitive human macrophage capping protein CAPG
Free Radic Biol Med (2021) 167, 81-93; doi:10.1016/j.freeradbiomed.2021.02.038

Mulnaes D, Koenig F, Gohlke H
TopSuite webserver: A meta-suite for deep learning-based protein structure and quality prediction
J. Chem. Inf. Model. (2021) 61(2), 548-553; doi:10.1021/acs.jcim.0c01202

Koch S, Seinen A-B, Kamel M, Kuckla D, Monzel C, Kedrov A, Driessen A J M
Single-molecule analysis of dynamics and interactions of the SecYEG translocon
FEBS J (2021) 288(7), 2203-2221; doi:10.1111/febs.15596

Bezrutczyk M, Zöllner NR, Kruse CPS, Hartwig T, Lautwein T, Köhrer K, Frommer WB, Kim J-Y
Evidence for phloem loading via the abaxial bundle sheath cells in maize leaves
The Plant Cell (2021) 33(3), 531–547; doi:10.1093/plcell/koaa055

Blomeier T, Fischbach P, Koch LA, Andres J, Minambres M, Beyer HM, Zurbriggen MD
Blue Light-Operated CRISPR/Cas13b-Mediated mRNA Knockdown (Lockdown)
Adv Biol (Weinh) (2021) 5(5):e2000307; doi:10.1002/adbi.202000307

Sanwald JL, Dobner J, Simons IM, Poschmann G, Stühler K, Üffing A, Hoffmann S, Willbold D
Lack of GABARAP-Type Proteins Is Accompanied by Altered Golgi Morphology and Surfaceome Composition
Int J Mol Sci (2021) 22(1): 85; doi:10.3390/ijms22010085. Epub 2020 Dec 23

Kim J-Y, Symeonidi E, Pang TY, Denyer T, Weidauer D, Bezrutczyk M, Miras M, Zöllner N, Hartwig T, Wudick MM, Lercher M, Chen L-Q, Timmermans MCP, Frommer WB
Distinct identities of leaf phloem cells revealed by single cell transcriptomics
The Plant Cell (2021) 33(3), 511–530, doi:10.1093/plcell/koaa060

Christie JM and Zurbriggen MD
Optogenetics in plants
New Phytol (2021) 229(6), 3108-3115; doi:10.1111/nph.17008. Epub 2020 Nov 28

2020

Stockner T, Gradisch R, Schmitt L
The role of the degenerate nucleotide binding site in type I ABC exporters
FEBS Lett (2020), 594(23), 3815-3838; doi:10.1002/1873-3468.13997

Baeumers M, Ruhnau K, Breuer T, Pannen H, Goerlich B, Kniebel A, Haensch S, Weidtkamp-Peters S, Schmitt L, Klein T
Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery
BMC Biol (2020) 18(1):200; doi:10.1186/s12915-020-00933-x

Loschwitz J, Olubiyi OO, Hub JS, Strodel B, Poojari CS
Computer simulations of protein–membrane systems
Prog Mol Biol Transl Sci (2020) 170, 273-403; doi:10.1016/bs.pmbts.2020.01.001

Oberleitner L, Poschmann G, Macorano L, Schott-Verdugo S, Gohlke H, Stühler K and Nowack Eva C. M.
The puzzle of metabolite exchange and identification of putative octotrico peptide repeat expression regulators in the nascent photosynthetic organelles of Paulinella chromatophora
Front Microbiol (2020), 11:607182; doi:10.3389/fmicb.2020.607182

Dimura M, Peulen T-O, Sanabria H, Rodnin D, Hemmen K, Hanke Ch A., Seidel C A. M. & Gohlke H
Automated and optimally FRET-assisted structural modeling
Nat Commun (2020) 11(1):5394; doi:10.1038/s41467-020-19023-1

Drews L, Zimmermann M, Westhoff P, Brilhaus D, Poss RE, Bergmann L, Wiek C, Brenneisen P, Piekorz RP, Mettler-Altmann T, Weber APM, Reichert AS
Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner
Dis Model Mech (2020) 13(10):dmm047134; doi:10.1242/dmm.047134

Chen H-Y, Chen Y-N, Wang H-Y, Lui Z-T, Frommer WB, Ho C-H
Feedback inhibition of AMT1 NH4+-transporters mediated by CIPK15 kinase
BMC Biol (2020) 18(1):196; doi:10.1186/s12915-020-00934-w

Braun CU, Hegemann JH, Mölleken K
Insights into a Chlamydia pneumoniae-specific gene cluster of membrane binding proteins
Front Cell Infect Microbiol (2020) 10:565808; doi:10.3389/fcimb.2020.565808

Kondadi AK, Anand R, Reichert AS
Cristae Membrane Dynamics - A Paradigm Change
Trends Cell Biol (2020) 30(12), 923-936; doi:10.1016/j.tcb.2020.08.008

J Friedl, MR Knopp, C Groh, E Paz, SB Gould, Herrmann JM, Boos F
More than just a ticket canceller: the mitochondrial processing peptidase tailors complex precursor proteins at internal cleavage sites
Mol Biology Cell (2020) 31:2657-2668

Muntjes K, Philipp M, Husemann L, Heucken N, Weidtkamp-Peters S, Schipper K, Zurbriggen MD, Feldbrugge M
Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
Front Microbiol (2020) 11:1384; doi:10.3389/fmicb.2020.01384

Anand R, Kondadi AK, Meisterknecht J, Golombek M, Nortmann O, Riedel J, Peifer-Weiß L, Brocke-Ahmadinejad N, Schlütermann D, Stork B, Eichmann TO, Wittig I, Reichert AS
MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes
Life Sci Alliance (2020) 3(10): e202000711. doi:10.26508/lsa.202000711

Ochoa-Fernandez R, Abel NB, Wieland F-G,Schlegel J,Koch L-A,Miller JB,Engesser R,Giuriani G,Brandl SM,Timmer J,Weber W,Ott T,Simon R,Zurbriggen MD
Optogenetic control of gene expression in plants in the presence of ambient white light
Nat Methods (2020) 17(7), 717-725; doi:10.1038/s41592-020-0868-y

Sanwald JL, Poschmann G, Stühler K, Behrends C, Hoffmann S, Willbold D
The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles
Cells (2020) 9(6):14689; doi:10.3390/cells9061468

Schepers B and Gohlke H
AMBER-DYES in AMBER: Implementation of fluorophore and linker parameters into AmberTools
J Chem Phys (2020) 152(22):221103; doi:10.1063/5.0007630

Reindl M, Stock J, Hussnaetter KP, Genc A, Brachmann A, Schipper K
A novel factor essential for unconventional secretion of chitinase Cts1
Front Microbiol (2020) 11:1529; doi:10.3389/fmicb.2020.01529

Löwe M, Kalacheva M, Boersma AJ, Kedrov A
The more the merrier: Effects of macromolecular crowding on the structure and dynamics of biological membranes
FEBS J (2020) 287(23), 5039-5067; doi:10.1111/febs.15429

Dobner J, Simons IM, Rufinatscha K, Hänsch S, Schwarten M, Weiergräber OH, Abdollahzadeh I, Gensch T, Bode JG, Hoffmann S, Willbold D
Deficiency of GABARAP but not its Paralogs Causes Enhanced EGF-induced EGFR Degradation
Cells (2020) 9(5):1296; doi:10.3390/cells9051296

Hoppen C and Groth G
Novel insights into the transfer routes of the essential copper cofactor to the ethylene plant hormone receptor family
Plant Signal Behav (2020) 15(2):1716512; doi:10.1080/15592324.2020.1716512

Hofmann A, Müller S, Drechsler T, Berleth M, Caesar K, Rohr L, Harter K, Groth G
High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1
Plants (2020) 9(3):304; doi:10.3390/plants9030304

Hänsch S, Spona D, Murra G, Köhrer K, Subtil A, Furtado AR, Lichtenthaler SF, Dislich B, Mölleken K, Hegemann JH
Chlamydia-induced curvature of the host-cell plasma membrane is required for infection
Proc Natl Acad Sci U S A (2020) 117(5), 2634-2644; doi:10.1073/pnas.1911528117

Knopp MR, Garg SG, Handrich M, Gould SB
Major changes in plastid protein import and the origin of the Chloroplastida
iScience (2020) 23(3):100896; doi:10.1016/j.isci.2020.100896

Steffens N, Beuter-Gunia C, Kravets E, Reich A, Legewie L, Pfeffer K, Degrandi D
Essential Role of mGBP7 for Survival of Toxoplasma gondii Infection
mBio (2020) 11(1):e02993-19; doi:10.1128/mBio.02993-19

Verma N, Dollinger P, Kovacic F, Jaeger K-E, Gohlke H
The membrane-integrated steric chaperone Lif facilitates active site opening of Pseudomonas aeruginosa lipase A
J Comp Chem (2020) 41(6), 500-512; doi:10.1002/jcc.26085

Viegas A, Dollinger P, Verma N, Seidel C, Gohlke H, Etzkorn M, Kovacic F, Jaeger K-E
Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation
Sci Rep (2020) 10(1):3578; doi:10.1038/s41598-020-60093-4

Kondadi AK, Anand R, Hänsch S, Urbach J, Zobel T, Wolf DM, Segawa M, Liesa M, Shirihai OS, Weidtkamp-Peters S, Reichert AS
Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner
EMBO Rep (2020) 21(3):e49776; doi:10.15252/embr.201949776

Mulnaes D, Porta N, Clemens R, Apanasenko I, Reiners J, Gremer
L, Neudecker P, Smits SHJ, Gohlke H
TopModel: Template-based protein structure prediction at low sequence identity using top-down consensus and deep neural networks
J Chem Theory Comput (2020) 16(3), 1953-1967; doi:10.1021/acs.jctc.9b00825

Kuhnert F, Stefanski A, Overbeck N, Drews L, Reichert AS, Stuhler K, Weber APM
Rapid Single-Step Affinity Purification of HA-Tagged Plant Mitochondria
Plant Physiol (2020) 182:692-706; doi:10.1104/pp.19.00732. Epub 2019 Dec 9

Gratz R, Brumbarova T, Ivanov R, Trofimov K, Tünnermann L, Ochoa-Fernandez R, Blomeier T, Meiser J, Weidtkamp-Peters S, Zurbriggen M, Bauer P
Phospho-mutant activity assays provide evidence 1 for alternative phospho-regulation pathways of the transcription factor FIT
New Phytol (2020) 225(1), 250-267; doi:10.1111/nph.16168. Epub 2019 Oct 13

2019

Trofimov K, Ivanov R, Eutebach M, Acaroglu B, Mohr I, Bauer P, Brumbarova T
Mobility and localization of the iron deficiency-induced transcription factor bHLH039 change in the presence of FIT.
Plant Direct. (2019) 3(12):e00190. doi:10.1002/pld3.190

Legewie L, Loschwitz J, Prescher M, Steffens N, Wang X, Smits SHJ, Schmitt L, Strodel B, Degrandi D, Pfeffer K
Biochemical and structural characterization of murine GBP7, a guanylate biniding protein with an unconventional C-terminal tail.
Biochem. J. (2019) 476(21): 3161-3182

Galle JN, Fechtner T, Eierhoff T, Römer W, Hegemann JH
A Chlamydia pneumoniae adhesin induces phosphatidylserine exposure on host cells.
Nat Commun (2019) 10(1):4644. doi: 10.1038/s41467-019-12419-8

Denay G, Schultz P, Haensch S, Weidtkamp-Peters S, Simon R
Over the rainbow: a practical guide for fluorescent protein selection in plant FRET experiments
Plant Direct. (2019) 3(12):e00189. doi: 10.1002/pld3.189

Barz B, Loschwitz J, Strodel B
Large-scale, dynamin-like motions of the human guanylate binding protein 1 revealed by multi-resolution simulations
PLoS Comput Biol. (2019) 7;15(10):e1007193. doi: 10.1371/journal.pcbi.1007193

Galle JN, Hegemann JH
Exofacial phospholipids at the plasma membrane: ill-defined targets for early infection processes.
Biol. Chem. (2019) 400(10):1323-1334. doi: 10.1515/hsz-2019-0187

Breda AS, Hazak O, Schultz P, Anne P, Graeff M, Simon R, Hardtke CS
A Cellular Insulator against CLE45 Peptide Signaling
Curr. Biol. (2019) 29, 1-8. doi.org/10.1016/j.cub.2019.06.037

Röder C, Vettore N, Mangels LN, Gremer L, Ravelli RBG, Willbold D, Hoyer W, Buell AK, Schröder GF
Atomic structure of PI3-kinase SH3 amyloid fibrils by cryo-electron microscopy.
Nat Commun. (2019) 10(1):3754. doi: 10.1038/s41467-019-11320-8

Kanonenberg K, Royes J, Kedrov A, Poschmann G, Angius F, Solgadi A, Spitz O, Kleinschrodt D, Stühler K, Miroux B, Schmitt L
Shaping the lipid composition of bacterial membranes for membrane protein production.
Microbial Cell Factories (2019), 18:131 doi 10.1186/s12934-019-1182-1

Krichel C, Möckel C, Schillinger O, Huesgen PF, Sticht H, Strodel B, Weiergräber OH, Willbold D, Neudecker P
Solution structure of the autophagy-related protein LC3C reveals a polyproline II motif on a mobile tether with phosphorylation site.
Sci Rep. (2019) 9(1):14167. doi: 10.1038/s41598-019-48155-8

Handrich MR, Garg SG, Sommerville EW, Hirt RP, Gould S
Characterization of the BspA and Pmp protein family of trichomonads
Parasit Vectors (2019) 12(1):406. doi: 10.1186/s13071-019-3660-z.

Berleth M, Berleth N, Minges A, Hänsch S, Burkart RC, Stork B, Stahl Y, Weidtkamp-Peters S, Simon R, Groth G
Molecular Analysis of Protein-Protein Interactions in the Ethylene Pathway in the Different Ethylene Receptor Subfamilies.
Front. Plant Sci. (2019) 10:726. doi: 10.3389/fpls.2019.00726

Bauemers M, Klose S, Brueser C, Haag C, Haensch S, Pannen H, Weidtkamp-Peters S, Feldbrügge M, Klein T
The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms.
Biol. Open (2019) 8, bio043422. doi:10.1242/bio.043422

Shanmugarajah K, Linka N, Gräfe K, Smits SHJ, Weber APM, Zeier J, Schmitt L
ABCG1 contributes to suberin formation in Arabidopsis thaliana roots.
Sci. Rep. (2019) 9(1):11381. doi: 10.1038/s41598-019-47916-9

Kater L, Frieg B, Berninghausen O, Gohlke H, Beckmann R, Kedrov A
Partially inserted nascent chain unzips the lateral gate of the Sec translocon.
EMBO Rep. (2019) 20(10):e48191. doi: 10.15252/embr.201948191

Hoppen C, Müller L, Hänsch S, Uzun B, Milić D, Meyer AJ, Weidtkamp-Peters S, Groth G
Soluble and membrane-bound protein carrier mediate direct copper transport to the ethylene receptor family
Sci. Rep. (2019) 9(1): 10715. doi: 10.1038/s41598-019-47185-6

Schott-Verdugo S, Müller L, Classen E, Gohlke H, Groth G
Structural model of the ETR1 ethylene receptor transmembrane sensor domain.
Sci. Rep. (2019)  9(1):8869. doi: 10.1038/s41598-019-45189-w

Jankowski S, Pohlmann T, Baumann S, Müntjes K, Devan SK, Zander S, Feldbrügge M
The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.
EMBO Rep. (2019) 20(9):e47381. doi: 10.15252/embr.201847381

Müller J, Pohlmann T, Feldbrügge M
Core components of endosomal mRNA transport are evolutionarily conserved in fungi.
Fungal Genet. Biol. (2019) 126: 12-16 

Klinker S, Stindt S, Gremer L, Bode JG, Gertzen CGW, Gohlke H, Weiergräber OH, Hoffmann S, Willbold D
Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.
J Biol Chem. (2019) 294(18):7388-7402

Schott-Verdugo S, Gohlke H
PACKMOL-Memgen: A simple-to-use generalized workflow formembrane-protein/lipid-bilayer system building.        
J. Chem. Inf. Model. (2019) 59(6):2522-2528. doi: 10.1021/acs.jcim.9b00269

Kanonenberg K, Smits S, Schmitt, L
Functional Reconstitution of HlyB, a Type I Secretion ABC Transporter, in Saposin-A Nanoparticles
Sci. Rep. (2019) 9(1):8436. doi: 10.1038/s41598-019-44812-0

Béthune J, Jansen R-P, Feldbrügge M, Zarnack K
Membrane-associated RNA-binding proteins orchestrate organelle-coupled translation.
Trends Cell Biol. (2019) 29: 178-188

Abdollahzadeh I, Hendriks J, Sanwald JL, Simons IM, Hoffmann S, Weiergräber OH, Willbold D, Gensch T
Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging.
Molecules (2019) 24(9). pii: E1833. doi: 10.3390/molecules24091833.

Khan I, Gratz R, Denezhkin P, Schott-Verdugo S, Angrand K, Genders L, Basgaran R, Fink-Straube C, Brumbarova T, Gohlke H, Bauer P, Ivanov R
Calcium-promoted interaction between the C2-domain protein EHB1 and metal transporter IRT1 inhibits Arabidopsis iron acquisition
Plant Physiol. (2019) 180(3):1564-1581. doi: 10.1104/pp.19.00163.

Schwarten M, Weiergräber OH, Petrović D, Strodel B, Willbold D
Structural Studies of Autophagy-Related Proteins.
Methods Mol Biol (2019) 1880: 17-56. doi: 10.1007/978-1-4939-8873-0_2

Spitz O, Erenburg IN, Beer T, Kanonenberg K, Holland IB, Schmitt L
Type I secretion systems - One mechanism for all?
Microbiol Spectr. (2019) 7(2). doi: 10.1128/microbiolspec.PSIB-0003-2018.

Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger K-E, Kovacic F
Expression, purification and crystallization of Pseudomonas aeruginosa
esterase PA2949, a bacterial homologue of human membrane esterase ABHD6.
Acta Cryst. F (2019) 75, 270-277

Werthebach M, Stewart FA, Gahlen A, Mettler-Altmann T, Akhtar I, Maas-Enriquez K, Droste A, Eichmann TO, Poschmann G, Stühler K, Beller M
Control of Drosophila Growth and Survival by the Lipid Droplet-Associated Protein CG9186/Sturkopf.
Cell Rep (2019) 26(13):3726-3740.e7. doi: 10.1016/j.celrep.2019.02.110.

Hoppen C, Müller L, Albrecht AC, Groth G
The NOP-1 peptide derived from the central regulator of ethylene signaling EIN2 delays floral senescence in cut flowers.
Sci Rep (2019) 9(1):1287. doi: 10.1038/s41598-018-37571-x.

Zavřel T, Faizi M, Loureiro C, Poschmann G, Stühler K, Sinetova M, Zorina A, Steuer R, Červený J
Quantitative insights into the cyanobacterial cell economy.
Elife (2019) 8;pii: e42508. doi: 10.7554/eLife.42508.

Reindl M, Hänsch S, Weidtkamp-Peters S, Schipper K
A Potential Lock-Type Mechanism for Unconventional Secretion in Fungi.
Int. J. Mol. Sci. (2019) 20(3):460. doi: 10.3390/ijms20030460

Simons IM, Mohrlüder J, Feederle R, Kremmer E, Zobel T, Dobner J, Bleffert N, Hoffmann S, Willbold D    
The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels.
Sci Rep. (2019) 9(1):526. doi: 10.1038/s41598-018-36717-1

Dreyer I, Spitz O, Kanonenberg K, Montag K, Handrich M, Ahmad S, Schott-Verdugo S, Navarro-Retamal C, Rubio-Meléndez M, Gomez-Porras JL, Riedelsberger J, Molina-Montenegro M, Succurro A, Zuccaro A, Gould S, Bauer P, Schmitt L, Gohlke H
Nutrient exchange in arbuscular mycorrhizal symbiosis from a thermodynamic point of view.
New Phytol. (2019) 222: 1043-1053

Haag C, Klein T, Feldbrügge M (2019)
Functional in vivo analysis of ESCRT components using model fungi.
In: Culetto E., Legouis R. (eds) The ESCRT Complexes. Methods in Molecular Biology, vol 1998, pp 251-271. Humana, New York, NY; DOI: 10.1007/978-1-4939-9492-2_19

2018

Möckel C, Kubiak J, Schillinger O, Kuehnemuth R, Della Corte D, Schröder GF, Willbold D, Strodel B, Seidel CAM, Neudecker P
Integrated NMR, Fluorescence and MD Benchmark Study of Protein Mechanics and Hydrodynamics.
J. Phys. Chem. B (2018) 123: 1453-1480

Aschenbroich J, Hussnaetter KP, Stoffels P, Langner T, Zander S, Sandrock B, Bölker M, Feldbrügge M, Schipper K
The germinal Centre kinase Don3 is crucial for unconventional secretion of chitinase Cts1 in Ustilago maydis.
Biochim. Biophys. Acta Proteins Proteom. (2018) 123: 1453-1480

Schnute B, Troost T, Klein T
Endocytic Trafficking of the Notch Receptor.
Adv Exp Med Biol. (2018) 1066:99-112, doi: 10.1007/978-3-319-89512-3_6

Mulnaes D and Gohlke H
TopScore: Using deep neural networks and large diverse data sets for accurate protein model quality assessment.
J. Chem. Theory Comput. (2018) 14: 6117-6126

Rösener NS, Gremer L, Reinartz E, König A, Brener O, Heise H, Hoyer W, Neudecker P, Willbold D
A D-enantiomeric peptide interferes with heteroassociation of amyloid-β oligomers and prion protein.
J. Biol. Chem. (2018) 293: 15748-15764

Ökmen B, Kemmerich B, Hilbig D, Wemhöner R, Aschenbroich J, Perrar A, Huesgen PF, Schipper K, Doehlemann G
Dual function of a secreted fungalysin metalloprotease in Ustilago maydis.
New Phytol. (2018) 220: 249-261

Owen MC, Kulig W, Rog T, Vattulainen I, Strodel B
Cholesterol Protects the Oxidized Lipid Bilayer from Water Injury: An All-Atom Molecular Dynamics Study.
J. Membr. Biol. (2018) 251: 521-534

Monné M, Daddabbo L, Gagneul D, Obata T, Hielscher B, Palmieri L, Miniero DV, Fernie AR, Weber APM, Palmieri F
Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate and dicarboxylates.
J. Biol. Chem. (2018) 293: 4213-4227

Kanonenberg K, Spitz O, Erenburg I, Beer T, Schmitt L
Type I secretion system - it takes three and a substrate.
FEMS Microbiol. Lett. (2018) 365(11). doi: 10.1093/femsle/fny094

Preisner H, Habicht J, Garg SG, Gould SB
Intermediate filament protein evolution and protists.
Cytoskeleton (2018) 75: 231-243

Milić D, Dick M, Mulnaes D, Pfleger C, Kinnen A, Gohlke H, Groth G
Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1.
Sci. Rep. (2018) 8: 3890. doi: 10.1038/s41598-018-21952-3

de Vries J and Gould SB
The monoplastidic bottleneck in algae and plant evolution.
J. Cell Sci. (2018) 131: jcs203414. doi: 10.1242/jcs.203414

Zander S, Müntjes K, Feldbrügge M
RNA live imaging in the model microorganism Ustilago maydis.
Methods Mol. Biol. (2018) RNA detection, ed. I. Gaspar, Humana Press, New York, 1649: 319-335.

Nowack ECM and Weber APM
Genomics-informed insights into endosymbiotic organelle evolution in photosynthetic eukaryotes.
Annu. Rev. Plant Biol. (2018) 69: 51-84

Heucken N and Ivanov R
The retromer, sorting nexins and the plant endomembrane protein trafficking.
J. Cell Sci. (2018) 131(2). pii: jcs203695. doi: 10.1242/jcs.203695

2017

Volkov O, Kovalev K, Polovinkin V, Borshchevskiy V, Bamann C, Astashkin R, Marin E, Popov A, Balandin T, Willbold D, Büldt G, Bamberg E, Gordeliy V
Structural insights into ion conduction by channelrhodopsin 2.
Science (2017) 358(6366), eaan8862. doi: 10.1126/science.aan8862

Waldera-Lupa DM, Etemad-Parishanzadeh O, Brocksieper M, Kirchgaessler N, Seidel S, Kowalski T, Montesinos-Rongen M, Deckert M, Schlegel U, Stühler K
Proteomic changes in cerebrospinal fluid from primary central nervous system lymphoma patients are associated with protein ectodomain shedding.
Oncotarget (2017) 8(66): 110118-110132

Berndt N, Seib E, Kim S, Troost T, Lyga M, Langenbach J, Haensch S, Kalodimou K, Delidakis C, Klein T
Ubiquitylation-independent activation of Notch signalling by Delta.
Elife. (2017) 6: e27346. doi: 10.7554/eLife.27346

Kessenbrock M, Klein SM, Müller L, Hunsche M, Noga G, Groth G
Novel Protein-Protein Inhibitor Based Approach to Control Plant Ethylene Responses: Synthetic Peptides for Ripening Control.
Front. Plant Sci. (2017) 8: 1528. doi: 10.3389/fpls.2017.01528

Lindenberg V, Mölleken K, Kravets E, Stallmann S, Hegemann JH, Degrandi D, Pfeffer K
Broad recruitment of mGBP family members to Chlamydia trachomatis inclusions.
PLoS One (2017) 12(9): e0185273. doi: 10.1371/journal.pone.0185273

Abdollahzadeh I, Schwarten M, Gensch T, Willbold D, Weiergräber OH
The Atg8 Family of Proteins-Modulating Shape and Functionality of Autophagic Membranes.
Front. Genet. (2017) 8: 109. doi: 10.3389/fgene.2017.00109

Mölleken K and Hegemann JH
Acquisition of Rab11 and Rab11-Fip2 - A novel strategy for Chlamydia pneumoniae early survival.
PLoS Pathog. (2017) 13(8): e1006556. doi: 10.1371/journal.ppat.1006556

Singer A, Poschmann G, Mühlich C, Valadez-Cano C, Hänsch S, Hüren V, Rensing SA, Stühler K, Nowack ECM
Massive protein import into the early evolutionary stage photosynthetic organelle of the amoeba Paulinella chromatophora.
Curr. Biol. (2017) 27: 2763-2773

Boeske A, Schwarten M, Ma P, Tusche M, Mötter J, Möller C, Neudecker P, Hoffmann S, Willbold D
Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.
Sci. Rep. (2017) 7: 5979. doi: 10.1038/s41598-017-06319-4

Hartings S, Paradies S, Karnuth B, Eisfeld S, Mehsing J, Wolff C, Levey T, Westhoff P, Meierhoff K
The DnaJ-like zinc-finger protein HCF222 is required for thylakoid membrane biogenesis.
Plant Physiol. (2017) 174: 1807-1824

Mergaert P, Kikuchi Y, Shigenobu S, Nowack ECM
Metabolic Integration of bacterial endosymbionts through antimicrobial peptides.
Trends Microbiol. (2017) 25: 703-712

McMillan B, Tibbe C, Drabek A, Seegar T, Blacklow S, Klein T
Structural basis for regulation of ESCRT-III complexes by Lgd.
Cell Rep. (2017) 19: 1750-1757

Martin WF, Tielens AGM, Mentel M, Garg SG, Gould SB
The physiology of phagocytosis in the context of mitochondrial origin.
Microbiol. Mol. Biol. Rev. (2017) 81(3): e00008-17. doi: 10.1128/MMBR.00008-17

Haag C, Pohlmann T, Feldbrügge M
The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.
PLoS Genet. (2017) 13(4):e1006734. doi: 10.1371/journal.pgen.1006734

Terfrüchte M, Reindl M, Jankowski S, Sarkari P, Feldbrügge M, Schipper K
Applying unconventional secretion in Ustilago maydis for the export of functional nanobodies.
Int. J. Mol. Sci. (2017) 18, 937. doi: 10.3390/ijms18050937

Lundquist PK, Mantegazza O, Stefanski A, Stühler K, Weber APM
Surveying the Oligomeric State of Arabidopsis thaliana Chloroplasts.
Mol. Plant. (2017) 10: 197-211.

Ivanov R and Bauer P
Sequence and coexpression analysis of iron-regulated ZIP transporter genes reveals crossing points between iron acquisition strategies in green algae and land plants.
Plant Soil (2017) 418: 61-73 2016

2016

Reimann S, Poschmann G, Kanonenberg K, Stühler K, Smits SH, Schmitt L
Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli.
Biochem. J. (2016) 473: 2471-2483

Fechtner T, Galle JN, Hegemann JH
The novel chlamydial adhesin CPn0473 mediates the lipid raft-dependent uptake of Chlamydia pneumoniae.
Cell Microbiol. (2016) 18(8): 1094-1105

Ball SG, Bhattacharya D, Weber APM
Pathogen to powerhouse. How did the precursors to the mitochondrion and the plastid evade host defense?
Science (2016) 351(6274): 659-660

Gould SB
Infection and the first eukaryotes.
Science (2016) 352(6289): 1065

Garg S and Gould SB
The role of charge in protein targeting evolution.
Trends Cell Biol. (2016) 26(12): 894-905

Lenders MHH, Beer T, Smits SHJ, Schmitt L
In vivo quantification of the secretion rates of the hemolysin A Type I secretion system.
Sci. Rep. (2016) 6: 33275. doi: 10.1038/srep33275

Preisner H, Karin EL, Poschmann G, Stühler K, Pupko T, Gould SB
The cytoskeleton of parabasalian parasites comprises proteins that share properties common to intermediate filament proteins.
Protist (2016) 167: 526-543

McMillan BJ, Tibbe C, Jeon H, Drabek AA, Klein T, Blacklow SC
Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein.
Cell Rep. (2016) 16(5): 1211-1217

Brumbarova T and Ivanov R
Differential Gene Expression and Protein Phosphorylation as Factors Regulating the State of the Arabidopsis SNX1 Protein Complexes in Response to Environmental Stimuli.
Front. Plant Sci. (2016) 7: 1456

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